縲梧椡闕芽曙縲阪↓髢「縺吶k繧オ繧、繝ャ繝ウ繝医く繝シ繝ッ繝シ繝峨後ご繝弱Β繝吶け繧ソ繝シ縲阪′蜷ォ縺セ繧後k遘醍�碑イサ謗。謚樒�皮ゥカ2莉カ 縲千�皮ゥカ蜷阪第椡闕芽曙繧イ繝弱Β繝吶け繧ソ繝シ縺ォ繧医k譁ー縺溘↑繧イ繝弱Β蟾・蟄ヲ繧キ繧ケ繝�Β縺ョ遒コ遶� <ul> 縲千�皮ゥカ蜷阪第椡闕芽曙繧イ繝弱Β繝吶け繧ソ繝シ縺ォ繧医k譁ー縺溘↑繧イ繝弱Β蟾・蟄ヲ繧キ繧ケ繝�Β縺ョ遒コ遶�<div class="jptitle5 縲千�皮ゥカ蛻�㍽縲大ソ懃畑蛻�ュ千エー閭樒函迚ゥ蟄ヲ縲千�皮ゥカ鬆伜沺隱イ鬘檎分蜿キ縲� https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-14J11456/" target="kaken" >14J11456 (KAKEN繝��繧ソ繝吶�繧ケ縺ァ隕九k��</a>縲千�皮ゥカ繧ュ繝シ繝ッ繝シ繝峨� <div class="jptitle4 譫ッ闕芽曙 / BAC / BGM / 繧イ繝弱Β繝吶け繧ソ繝シ / RecA / 繝医Λ繝ウ繧ケ繧ク繧ァ繝九ャ繧ッ繝槭え繧ケ縲千�皮ゥカ謌先棡縺ョ讎りヲ√� <div class="jptitle4 蟾ィ螟ァDNA譁ュ迚��驕コ莨晏ュ先桃菴懈橿陦薙�繧イ繝弱Β讖溯�隗」譫舌↓荳榊庄谺�縺ェ蝓コ逶、謚陦薙〒縺ゅj縲√ご繝弱Β隗」隱ュ縺碁イ繧縺ォ縺、繧後※縺セ縺吶∪縺吶◎縺ョ驥崎ヲ∵ァ縺悟「励@縺ヲ縺�k縲�Mb繧ケ繧ア繝シ繝ォ縺ョ驕コ莨晏ュ先桃菴懊′蜿ッ閭ス縺ェ譫ッ闕芽曙��BGM�峨ご繝弱Β繝吶け繧ソ繝シ縺ォ逹逶ョ縺励∵ャ。荳紋サ」縺ョDNA謾ケ螟峨す繧ケ繝�Β縺ョ遒コ遶九�縺溘a荳玖ィ假シ偵▽縺ョ遐皮ゥカ繧貞ョ滓命縺励∝、壽ァ倥↑驕コ莨晏ュ先桃菴懈ウ輔d繝医Λ繝ウ繧ケ繧ク繧ァ繝九ャ繧ッ繝槭え繧ケ縺ク縺ョ蠢懃畑謚陦薙�謨エ蛯吶∵隼濶ッ蝙毅GM繝吶け繧ソ繝シ縺ョ讒狗ッ峨∝ュヲ陦鍋�皮ゥカ縺ク縺ョ蠢懃畑繧帝#謌舌@縺溘よ悽謚陦薙�縲∫黄雉ェ逕溽肇繧�ココ蟾・繧イ繝弱Β蜷域�縲√ヨ繝ゥ繝ウ繧ケ繧ク繧ァ繝阪す繧ケ縲√ご繝弱Β讖溯�隗」譫舌↑縺ゥ縲�⊆莨晏ュ先桃菴懊r縺翫%縺ェ縺�肇讌ュ繝サ蟾・蟄ヲ繝サ蟄ヲ陦薙�縺ゅi繧�k蛻�㍽縺ク縺ョ蠢懃畑縺梧悄蠕�〒縺阪k縲�<br />�托シ弱く繧キ繝ュ繝シ繧ケ隱伜ー弱き繧サ繝�ヨ繧堤畑縺�※RecA隱伜ー主梛譫ッ闕芽曙譬ェ��inducible recA expression BGM vector: iREX�峨r讒狗ッ峨@縺溘JREX縺ォ縺翫¢繧騎ecA縺ョ逋コ迴セ縺ッ繧ュ繧キ繝ュ繝シ繧ケ縺ォ繧医j蜴ウ蟇�↓蛻カ蠕。縺輔l縺ヲ縺翫j縲∬ェ伜ー取凾縺ォ縺ッ縺薙l縺セ縺ァ騾壹j繝励Λ繧ケ繝溘ラ繧ВAC-DNA繧偵け繝ュ繝シ繝九Φ繧ー縺ァ縺阪k縺薙→繧堤、コ縺励◆縲ゅく繧キ繝ュ繝シ繧ケ髱槫ュ伜惠荳九〒縺ッ縲∵里蟄倥�BGM繝吶け繧ソ繝シ縺ァ隕九i繧後※縺�◆蝓ケ鬢贋クュ縺ョ逶ク蜷檎オ�鋤縺医r謚代∴繧九%縺ィ縺ァ繧医j螳牙ョ壹↓繧、繝ウ繧オ繝シ繝医r菫晄戟縺ァ縺阪∝セ捺擂縺ョ繧キ繧ケ繝�Β縺ァ縺ッ讒狗ッ峨′髮」縺励°縺」縺溯、�焚縺ョ鬘樔シシ驟榊��医Ξ繝昴�繧ソ繝シ驕コ莨晏ュ千ュ会シ峨r蜷ォ繧繧医≧縺ェ隍�尅縺ェ繝医Λ繝ウ繧ケ繧ク繝シ繝ウ縺ォ繧ょソ懃畑縺ァ縺阪k縺薙→繧堤、コ縺励◆縲�<br />�抵シ撮GM繝吶け繧ソ繝シ縺ョ螳溽畑諤ァ繧堤、コ縺吶◆繧√↓縲。AC繧ッ繝ュ繝シ繝ウ縺ョ騾�ス阪�騾」邨先桃菴懊r鬧�スソ縺励※繝槭え繧ケ豌エ譽イ蝙句羅隕壼女螳ケ菴馴⊆莨晏ュ舌ご繝弱Β鬆伜沺縺ョ蜀肴ァ狗ッ峨r縺翫%縺ェ縺�`b繧ケ繧ア繝シ繝ォ縺ョ騾」邨仙梛繝医Λ繝ウ繧ケ繧ク繝シ繝ウ縺ョ讒狗ッ峨r隧ヲ縺ソ縺溘�252 kb縺セ縺ァ縺ッ蜀肴ァ狗ッ峨〒縺阪※縺�◆繧ゅ�縺ョ縲√し繧、繧コ縺ョ螟ァ縺阪↑BAC-DNA縺ョ繧ッ繝ュ繝シ繝九Φ繧ー縺ォ縺翫>縺ヲ譁ー縺溘↑隱イ鬘後′譏弱i縺九↓縺ェ縺」縺溘ゅ@縺九@縺ェ縺後i蛻・縺ョ逋コ螻戊ェイ鬘後↓縺翫>縺ヲ縲。GM繝吶け繧ソ繝シ縺ァ縺ョ驕コ莨晏ュ先桃菴懊d繝医Λ繝ウ繧ケ繧ク繧ァ繝九ャ繧ッ繝槭え繧ケ縺ョ隗」譫舌r鬧�スソ縺吶k縺薙→縺ォ繧医j縲∝�繧√※豌エ譽イ蝙句羅隕壼女螳ケ菴馴⊆莨晏ュ舌�逋コ迴セ蛻カ蠕。鬆伜沺縺ョ蜷悟ョ壹↓謌仙粥縺励◆縲� 縲� 縲千�皮ゥカ蜷阪第椡闕芽曙繧イ繝弱Β繝吶け繧ソ繝シ(BGM繝吶け繧ソ繝シ)縺ク縺ョ蜉ケ譫懃噪縺ェ繧ッ繝ュ繝シ繝九Φ繧ー謚陦薙�髢狗匱 縲千�皮ゥカ莉」陦ィ閠�鷹≡蟄� 逵滉ケ� 譚ア莠ャ蟾・讌ュ螟ァ蟄ヲ 螟ァ蟄ヲ髯「繝サ逕溷多逅�キ・蟄ヲ遐皮ゥカ遘� 蜉ゥ謨� https://nrid.nii.ac.jp/ja/nrid/1000010399694/